草棉花花瓣不同提取物对实验性肝损伤小鼠的保护作用

Studies on the hepatoprotective effect of Gossipium hirsutum extract on the acute experimental hepatitis

目的:研究草棉花花瓣采用不同工艺提取的产物提取物Ⅰ和提取物Ⅱ(FGF-Ⅰ、FGF-Ⅱ)对扑热息痛引起的实验性肝损伤是否有保护作用。方法:选用昆明种小鼠,采用腹腔注射扑热息痛的方法造成肝损伤动物模型;观察FGF-Ⅰ和FGF-Ⅱ对血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)水平的影响。结果:FGF-Ⅰ和FGF-Ⅱ对小鼠血清ALT、AST活性均有降低作用。FGF-Ⅰ对肝组织SOD、GSH-PX活性没有影响,但可以降低肝组织MDA含量。FGF-Ⅱ可以增加肝组织SOD活性、降低MDA含量、增加GSH-PX活性。结论:FGF-Ⅰ和FGF-Ⅱ均有一定的保肝作用,但是FGF-Ⅱ作用优于FGF-Ⅰ。

Objective：To investigate the hepatoprotective effect of Flos Gossypium herbaceum extracts（FGF-Ⅰ,FGF-Ⅱ） on the acute experimental hepatitis induced by paracetamol（PAR）.Methods：Experimental hepatitis animal model were established in mice by single intraperitoneal injection of PAR（180 mg/kg）.The alanine aminotransferase（ALT）,aspartate transferase（AST） level in serum and malondialdehyde（MDA）,super oxide dismutase（SOD）,glutathione peroxidese（GSH-PX） activities in liver homogenate were examined.Results：In experimental hepatitis animal model induced by PAR,FGF-Ⅰ（35,70 mg·kg） and FGF-Ⅱ（35,70 mg/kg） were found to significantly decrease the serum transaminase activities significantly（P〈0.05）.Meanwhile,FGF-Ⅰ（70,35 mg/kg） had no significant effect on SOD,GSH-PX（P〉0.05）.But it can decreased the MDA content（P〈0.05）.FGF-Ⅱ also decreased MDA content（P〈0.05）,and prevented the reduction of SOD,GSH-PX activities in liver（P〈0.01~0.05）.Conclusion：FGF-Ⅱ and FGF-Ⅰ show significant protective action on liver injury in mice.