大鼠肾缺血再灌注损伤时脑组织自由基的变化

The change of free radical in brain after renal ischemia-reperfusion injury

目的:观察探讨大鼠肾缺血再灌注(I/R)损伤过程中脑组织自由基的变化规律,探讨其可能的机制。方法:40只健康雄性SD大白鼠随即分为对照组和肾I/R组,检测和比较肾缺血30、60、90、120 min再灌注60 min后血浆尿素氮(BUN)、肌酐(Cr)、肾系数、血浆、肾和脑组织中丙二醛(MDA)和谷胱苷肽过氧化物酶(GSH-PX)的含量。结果:与对照组相比,在肾I/R后血浆的BUN、Cr含量、肾系数及血浆、肾和脑组织中MDA含量均升高(P<0.05),肾I/R组血浆、肾和脑组织GSH-PX活性降低(P<0.05)。结论:肾I/R损伤后,血浆和脑组织中代谢产物积聚同时自由基生成增多,提示肾I/R损伤后神经系统功能的改变可能与其相关。

Objective：To determine the changing patterns of free radical in brain after renal ischemia-reperfusion（I/R） injury.Methods：Forty healthy male SD rats were divided randomly into control group and renal I/R injury group（I/Rgroup）,the contents of BUN,Cr,GSH-PX and MDA in plasma and GSH-PX and MDA in brain were determined.Results：Compared with control group,after renal I/R,the contents of BUN,Cr and MDA increased（P〈0.05）,while the activity of GSH-PX dropped（P〈0.05） of plasma,kidney and brain in the IR group.Conclusion：Renal I/R injury can lead to the accumulation of metabolism and the creation of free radical in kidney and the tissues of brain,which indicates that the dysfunction of nerve system may be related to the change of free radical in cerebrospinal fluid after I/R injury in renal.