皮片在不同保存液中短期储存活力变化的临床研究

Effect of methods of short-term tissue storage on split-skin viability at 4℃ refrigeratory

目的:探讨在4℃条件下短期皮片储存方法对皮片活力的影响,以期找到一种简便、有效、实用的短期储皮方法,利于临床应用。方法:在4℃条件下,分为生理盐水、红霉素盐水和复方氨基酸(18AA)红霉素混合液3个实验组,普通冰箱储存手术中剩余皮片,在储存1、3、5、7 d共4个时间点分别采用氢电子受体氯化碘硝基四唑(in-donitrotetrazolium chloride,INT)法测定皮片活力值。结果:4℃条件下短期储存皮片活力测定比较,复方氨基酸红霉素混合液组优于红霉素盐水组和生理盐水组。3个处理组间皮片活力及不同时间皮片活力的差异有统计学意义(P<0.05)。结论:将术中剩余皮片浸泡在复方氨基酸红霉素混合液内普通冰箱短期储存是一种简便、有效、实用的方法,在临床中有实用价值。

Objective：To discuss effect of various methods of short-term tissue storage on split-skin viability at 4℃ to find a kind of clinic storage of skin and the viabilitiy of skins stored at correspondent condition.Methods：Three experiment groups investigated for storing hominine split-skin at 4℃ were 0.9% sodium chloride solution,0.9% sodium chloride solution plus erythromycin injection and compound amino acid solution（18AA） plus erythromycin injection.A new assy,indonitrotetrazolium chloride was applied to evaluate skin viability at day 1,3,5 and 7 during storage respectively.Results：Skin viability was very different in three solutions.The effect of compound amino acid solution plus erythromycin injection was superior to 0.9% sodium chloride solution and 0.9% sodium chloride solution plus erythromycin injection（P=0.000）.Conclusion：The method of compound amino acid solution plus erythromycin injection which replaces medium routinely may be optimal method of storing skin at 4℃,it was very simple and convenient.