小鼠乳鼠心肌细胞的原代培养

The primary cultures of newnatal rat of mouse cardiacmyocytes

目的：观察原代培养的小鼠乳鼠心肌细胞的形态特征。方法：Ⅱ型胶原酶、胰蛋白酶消化心脏组织，差速贴壁分离法。结果：12h心肌细胞基本贴壁，第2天可长成单层，第3～4天细胞伸出伪足相互接触交织成网，逐渐形成细胞簇或细胞单层，搏动呈同步性，收缩明显有力，搏动频率为30～120次／min。结论：用Ⅱ型胶原酶、胰蛋白酶分离培养小鼠乳鼠心肌细胞可获得形态、活力良好的心肌细胞。

Objective： To Observe the morphological characteristics of the cultured cardiomyocytes. Methods： The hearts were digested by collagenase type Ⅱ , trypsin and D Nase Ⅰ. The cell suspension was preplated to reduce the fibroblast content. Results： The cells spread over the first day and developed their characteristic morphology by day 3 or 4. They were arranged in loose and interlacing networks of single cells. The cells beat spontaneously at 30-110 per minute. Conclusion： It can obtain cardiomyocytes with well morphology and spontaneous beating with collagenase type Ⅱ , trypsinandDNase Ⅰ.