锌对砷中毒小鼠肝肾脂质过氧化影响的实验研究

Experimental study of Zinc affect Lipid peroxidation of arsenic on liver and kidney in mice

目的:探讨砷对小鼠肝、肾脂质过氧化的影响及锌的拮抗作用。方法:健康昆明种小鼠60只,随机分为阴性对照组、阳性对照组、砷+低锌组、砷+中锌组及砷+高锌组共5组,采用亚慢性毒性实验的方法,经口连续灌胃6周,实验结束后处死小鼠,分别测定小鼠肝、肾组织中脂质过氧化产物丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性,同时观察锌干预对染砷小鼠体内抗氧化水平的影响。结果:随着砷染毒剂量的增加,小鼠肝、肾组织中MDA含量显著增加,SOD、GSH-Px活性显著降低。锌能抑制MDA生成量,提高SOD、GSH-Px活性。结论:砷可致机体脂质过氧化,锌可拮抗其脂质过氧化作用。

Objective： To investigate the effect of arsenic on lipid peroxidation in mice liver and observe the intervention effect of zinc supplement. Methods： Sixty health Kunmingmice were divided into five groups （negative control group, the positive control group, three zinc intervention group）, a sub-chronic toxicity test methods, oral gavage for six weeks until the end of the experiment were killed. Lipid peroxidation malondialdehyde （MDA） content, superoxide dismutase （SOD）, activity of glutathione peroxidase （GSH-Px） in mice liver and kidney were measured. At the same time observed after the intervention of zinc in mice with arsenic levels of anti-oxidation. Results： The results showed that with the increases of exposure levels of arsenic, the contents of MDA was significantly increased and the activities of SOD and GSH-Px were significantly decreased. Zinc could decrease the contents of MDA and increase the activities of SOD and GSH-Px significantly. Conclusion： The arsenic could induce lipid peroxidation in mice and zinc could inhibit the lipid peroxidation result in exposed to arsenic.