正交试验优选微波法提取板蓝根总有机酸的工艺

Optimization of the Microwave-assisted Extraction of Total Organic Acids from Isatis indigotica by Or-thogonal Test

目的:优选微波法提取板蓝根中总有机酸的工艺。方法:以总有机酸的提取率为指标,应用酸碱滴定法测定提取物中总有机酸的含量,采用正交试验法对提取过程中的提取溶剂倍量、微波功率、微波辐照时间3个因素进行优选。结果:最佳提取条件为使用15倍药材量的水为溶剂,微波功率为522W,提取时间为20min,在此条件下总有机酸提取率达4.83%,接近水煎煮2h的提取率。结论:本方法具有快速、方便、节能、提取效率高等优点,值得推广应用。

OBJECTIVE： To optimize the microwave - assisted extraction of total organic acids from Isatis indigotica. METHODS： With the dissolution rate of total organic acids as indexes, the content of total organic acids was determiend by acid- base titration and the three main factors including volume of solvent, microwave power and extraction time in the extraction process were optimized by orthogonal test. RESULTS： The optimal extraction process is to add 15 times amount of water and extract at a microwave power of 522 W for 20 minutes. Under the above condition, the extraction rate was 4.83%, close to the extraction yield achieved by water decoction for 2 h. CONCLUSION： The microwave- assisted extraction of total organic acids from Radix Isatidis is characterized by rapid, simple, efficient and energy- saving etc, thus deserving to be be popularized.