罗布麻叶指纹图谱研究和品质评价

Fingerprint and Quality Evaluation of Apocynum Venetum Leaves

目的:建立罗布麻叶的高效液相色谱指纹图谱,测定样品中槲皮素含量,对商品罗布麻叶的品种和质量进行评价。方法:色谱柱为汉邦C18(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸(梯度洗脱),检测波长为360nm,流速为0.9mL·min-1。结果:槲皮素精密度良好(RSD=0.21%,n=6);罗布麻叶溶液重现性良好(RSD=1.8%,n=6),且10h内较为稳定。结论:本研究为罗布麻叶药材的鉴定和品质评价提供了科学依据。

OBJECTIVE： To establish the HPLC fingerprints of Apocynum Venetum leaves, determine the content of Quercetin in the sample and evaluate the variety and quality of commercial Apocynum Venetum leaves. METHODS： Quercetin was separated on Zorbarx - C18（250mm×4.6mm,5μm）, and the mobile phase consisted of methanol and 0.4% H3PO4（gradient elution） at a flow rate of 0.9 mL · min^ -1 with the detective wavelength set at 360 nm. RESULTS： The pre- cision of Quercetin was satisfactory（RSD = 0.21%, n = 6）, and the solution of Apocynum Venetum leaves was reproducible and stable within 10 hours. CONCLUSION： This study serves as a scientific basis for the identification and quality evalution of Apocynum Venetum leaves.