摘要
目的:建立以高效液相色谱法测定胃炎口服液中黄芩苷含量的方法。方法:色谱柱为ZorbaxSB—C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(50:50:0.2),检测波长为276nm。结果:黄芩苷进样量在0.065~0.520μg范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均回收率为99.79%,RSD=1.28%(n=6)。结论:本方法简便、准确;可用于胃炎口服液的质量控制。
OBJECTIVE: To establish an HPLC method to determine the content of Baicalin in Oral liquid for gastritis. METHODS: Zorbax SB- C18(150 mm × 4.6 mm, 5μm) was used as chromatographic column, and the mobile phrase consisted of methanol- water- phosphoric acid(50 : 50 : 0.2) with detection wavelength set at 276 nm.RESULTS: The calibration curve of baicalin was in good linearity over the range of 0.065--0. 520μg (r = 0.999 8). The average recovery of Baicalin was 99.79% with RSD at 1.28% (n = 6) .CONCLUSION: The method is simple and accurate and applicable for the quality control of Oral liquid for gastritis.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第24期1889-1890,共2页
China Pharmacy