产后抑郁与雌激素受体β基因单核苷酸多态性的关联研究

Association study using single nucleotide polymorphisms(SNPs) in the estrogen receptor (ERβ) gene for postpartum depression

目的探讨产后抑郁可能存在的雌激素受体β(Estrogen Receptor Beta,ERβ)基因遗传易感性。方法从刚进行过临床试验的746例连续整群抽样的产妇整体中,随机选择产后抑郁者68例和非产后抑郁者93例,采用聚合酶链反应-限制性酶切多态性(PCR-RFLP)的方法进行ERβ的2个SNPs(rs1256030和rs3020444)基因分型。采用病例-对照关联分析和两点单体型关联分析获得产后抑郁的遗传易感性。结果ERβ基因rs3020444(T/C)多态"T/T"基因型与产后抑郁关联,导致产妇患产后抑郁的风险是其它基因型的2.91(1.22-6.91)倍(P<0.05);"T"等位基因导致产妇患产后抑郁的风险是"C"等位基因的2.72(1.19-6.18)倍(P<0.05)。未发现rs1256030(C/T)的等位基因或基因型与产后抑郁存在关联。基于rs1256030(C/T)和rs3020444(T/C)的单体型"C-C"对产后抑郁具有保护作用,OR=0.306(0.113-0.827),P=0.019。结论ERβ基因有可能参与了产后抑郁的发病。但需要扩大样本进一步验证。

Objective： To investigate the ERβ gene susceptivity for postpartum depression. Methods： Sixty- eight cases （depressed women postpartum ） and 93 control （non - depressed women postpartum ） selected from the 746 continuity who had joined the preceding clinical test were genotyped by ERβ gene two SNPs （rs1256030 and rs3020444） with polymerase chain reaction- restriction fragment length polymorphism （ PCR - RFLP）. Case - control association and two - point haplotype association with postpartum depression were performed. Results： The genotype ＂ T/T＂ according to ERβ gene rs3020444 （T/C）polymorphism was found to be associated with postpartum depression. The case who had the ＂ T/T＂ genotype developed postpartum depression 2.91 （ 1.22 - 6.91 ） times the other genotypes（ P 〈 0. 05 ）. The case who had the ＂T＂ allele developed postpartum depression 2.72 （ 1.19 - 6.18 ） times the ＂C＂ allele （ P 〈 0.05 ）. The haplotype ＂ C- C＂ based on rs1256030（C/T） and rs3020444 （T/C）polymorphisms had a protective effect on postpartum depression, OR =0.306（0. 113 -0.827） ,P =0.019. However, the rs1256030（C/T） alleles or genotypes were not associated with postpartum depression. Conclusion：ERβ gene may join in the pathogenesis of postpartum depression. But a large sample is needed to testify the finding.