锰过氧化物酶产生菌的发酵优化及酶学性质研究

Screening,cultivation and crude enzyme characterization of manganese peroxidase from white rot fungus

采用愈创木酚初筛平板从无锡惠山、龙山等地筛选出6株阳性菌株,通过摇瓶复筛从中得到一株产MnP活性较高的菌株SYBC-M3;同时进行部分发酵产酶条件的研究。在此基础上,对得到的MnP进行了粗酶的部分酶学性质研究。结果表明,当培养基中豆粕20 g/L、蛋白胨10 g/L、Tween-200.5 g/L、Mn2+1.5 mmol/L,起始pH 5.0时,MnP的活力有较大程度的提高,可达1 775 U/L;其最适作用温度55℃,最适pH 5.0;同时其温度和pH稳定性也较好;pH 9.0下24 h还可保持90%以上的活性。

Six fungi were screened positive in guaiacol - containing PDA plates from nature. Then a high yield producing manganese peroxidase （MnP） strain （ SYBC - M3 ） was obtained by fermenting the 6 positive strains. The effects of different fermentation conditions on MnP production was studied primitively from SYBC - M3, and the properties of MnP the researched. The results showed that the activation of MnP can reach 1 775 U/L in the culture with soybean meal 20 g/L, peptone 10 g/L, Tween - 20 0.5 g/L, Mn^2＋ 1.5 mmol/L. The optimal temperature and pH were 55 ℃ and 5.0 respectively. Furthermore, the thermal and pH stabilities of MnP were pretty good. It had a high activity by maintaining in pH 9.0 for 24 h.