工农业废料玉米浆和豆饼粉浓度对松茸胞外多聚物产率的影响

The effect of industrial and agricultural waste corn steep liquor and soybeanl meal on tricholoma matsutake exo-biopolymer production

利用工农业废料作为廉价培养基培养名贵药食用真菌松茸,从而获得其有效成分胞外多聚物。通过单因素试验选取试验因素与水平,在单因素试验的基础上,以培养基中玉米浆浓度和豆饼粉浓度为响应因子,胞外多聚物产率为响应值,做两因素五水平的中心组合试验。培养基中玉米浆浓度为9.07%,豆饼粉浓度为10.43%时,胞外多聚物产率最大可达36.20 g/L,比传统试验试剂培养基提高了2.6倍,这对实现新生物技术产业化具有重要的理论与实践意义。

To evaluate the capability of industrial and agricultural waste being low cost substrates on the exo-biopolymer production by cultivating the most valuable and high-class edible fungus Tricholoma matsutake. The effect of corn steep liquor and soybean meal on the exo-biopolymer production was investigated by means of single factor tests and response surface methodology （RSM） based on a five-level two-factor central composite design. Experiment factors and levels were first selected by single factor tests. On the basis of this, the concentration of corn steep liquor and soybean meal were chosen as the factors in RSM and each factor in the design was studied at five different levels. Optimized values of the factors were determined：corn steep liquor 9.07 %, soybean meal 10.43 % and a maximum exo-biopolymer production 36.2 g/L, which was 2.6 times higher than the costly traditional medium. It was low cost by using cheap and abundant source of industrial and agricultural waste to replace the experimental reagents as a medium for cultivating Tricholoma matsutake, and has an important theoretical and practical meaning to the realization of the new biotechnology industry.