摘要
建立食品中糖精钠、甜蜜素、安赛蜜和苯甲酸的高效液相色谱-质谱（HPLC-MS）检测方法。色谱柱为Spherigel C18（5μm，4．6×200Mm），流动相为：（A）甲醇-（B）甲酸-三乙胺缓冲盐，梯度洗脱；电喷雾负离子（ESI^-）采集模式，流速为1．0mL／min，进样量为10μL；以华法林钠为内标，质谱定性定量；安赛蜜、糖精钠、甜蜜素、苯甲酸的线性范围分别为：5．4～135，5．4～135，7．4～111，10～150μg／mL，加样回收率为93．19％～100．90％，RSD为1．05％～2．04％．该方法具有选择性好、分析时间短、定性定量准确等优点，可用于果冻等食品中的4种目标物定性定量检测。
Established a HPLC - ESI/MS method for Simultaneous determination of saccharin sodium dehydrate, acesulfame, sodium cyclamate, Benzoic acid. Column： Spherigel C18 （5 μm, 4.6×200 mm）. Mobile phases ： （A） methanol - （B） buffer methanoic acid - Triethylamine （ pH = 4.50 ）, Gradient elution. Flow rate ： 1.0 mL/min, and 0.2 mL/min to MS ionization. By using warfarin sodium as internal standard and using qualitative and quantitative MS determination, the 1 inear ranges of Saccharin sodium dehydrate, acesulfame, sodium cyclamate, Benzoic acid were 5.4 - 135.0, 5.4 - 135.0, 7.4 - 111, 10 - 150 μg/mL, respectively. The average recoveries of saccharin sodium dehydrate, acesulfame, sodium cyclamate, benzoic acid were between 93.19% - 100, 90 % ,and RSD were 1.05% - 2.04%. The method can be used to determine the contents in jelly foods, which is fine selective, short time detection, accurate in qualitative and quantitative determining
出处
《食品与机械》
CSCD
北大核心
2008年第4期111-114,共4页
Food and Machinery
