摘要
采用多因素正交试验确定了酿酒酵母和糖化酵母原生质体形成和再生的最佳条件。酿酒酵母对数前期细胞在１％蜗牛酶、０．１％的巯基乙醇（ＭＥ）、０．１％ＥＤＴＡＮａ２，３０℃条件下，处理４０ｍｉｎ；糖化酵母对数前期细胞在２％蜗牛酶、０．１％的巯基乙醇（ＭＥ）、０．１％ＥＤＴＡＮａ２，３０℃条件下，处理４０ｍｉｎ，酿酒酵母原生质体形成率和再生率分别为８９．１％和１０．８％，原生质体形成率×再生率值最大（９．６％）。糖化酵母原生质体形成率和再生率分别为９１．４％和１０．３％，原生质体形成率×再生率值最大（９．４％）。
orthogonal test was used to determine the optimal conditions for protoplastformation and regeneration of S.cerevisias and S.diastaticus. The propduct of the frequency of protoplast formation of S.cerevisias (89.1%) multiplied by the frequency of protoplast regeneration (10.8%) is maxist (9.6%) ,which consists of early exponential phase cells, 1% snail enzyme,0.1% 2-mercaptoethanol, 0.1% EDTANa2, 30℃ and treatment for 40min. The product of the frequency of protoplast formation of S.diastaticus (91.4%)multiplied by the frequency of protoplast regeneration (10.3%) is maxist(9.4%),which consists of early exponential phase cells, 2% snail enzyme,0.1% 2-mercaptoethanol, 0.1% EDTANa, 30℃ and treatment for 40min.
出处
《莱阳农学院学报》
1997年第4期245-248,共4页
Journal of Laiyang Agricultural College
