黑曲霉菌丝体真空冷冻干燥的研究

Preservation of the Mycelium of Aspergillus Niger by Freeze Drying

本文研究了黑曲霉(ATCC16404)菌丝体真空冷冻干燥的保藏方法,并从培养基和保护剂对冻干菌的影响进行了研究,初步确定了高活菌数的增殖培养基。比较了不同保护剂对菌丝体成活率的影响,确定了最佳保护剂,活菌数达到109cfu/g。

In this paper, the preservation of the mycelium of Aspergillus niger by freeze drying was introduced ,and the effects of the cultural medium and freeze-drying protectant were investigated. The enrichment culture medium for Aspergillus niger with high viable cells number and the best freeze-drying protectant were determined, with which the viable cells number of Aspergillus niger treated by freeze drying were 10^9cfu/g.