大豆茎顶端分生组织石蜡切片的制备

Manufaction of Paraffin Section on Apical Meristem of Soybean

为探讨大豆茎顶端分生组织石蜡切片的制作方法,观察大豆茎顶端发育过程的微观结构变化。将大豆顶芽经固定、脱水、透明、浸蜡、包埋、切片、染色等一系列过程,获得其纵切片。结果表明:生长锥顶端、叶原基、花原基等分裂活跃的部位染色较深,髓分生组织部位染色较浅;在营养生长阶段,叶原基依次从茎顶端分生组织底部发育,逐渐变大;在生殖生长阶段,顶端变成伸长的圆顶形,然后开始分化各轮花器官。研究结果可为深入观察大豆茎顶端分生组织解剖结构及分生组织发育的分子生物学研究提供基础。

In order to obtain more microstructure information on development of soybean, the methods for apical meristem sections were studied in the paper. Based on the process of fixation, dehydration, transparency, saturated paraffin, embedding, section and dye, straight- sections of the shoot apical meristem of soybean were obtained. The results from this study indicated that the regions splitting actively, such as the top of apical meristem, leaf primordia and flower primordia, were dyed deeply. At the same time, pith meristem zone was dyed lightly. At vegetative growth stage,leaf pfimordia were found at the basal part of apical meristem, and they became bigger gradually. At reproductive growth stage, the dome-liked apical meristem became longer slightly, then each of floral primordia began to appear in turn. The results could provide the base for further investigation on anatomical structure and developmental biology of shoot apical meristem in soybean.