OX-42和Fos蛋白在海人酸致癫大鼠前脑中早期的表达变化

Early changes of expressions of OX-42 and Fos proteins in forebrain of rats with kainic acid-induced seizure

目的:研究大鼠在海人酸(KA)导致癫痫发作早期前脑内小胶质细胞的变化及其与神经元的关系.方法:应用免疫组织化学法单标分别显示前脑内OX-42和Fos蛋白表达的时间规律,并用免疫组织化学双重标记显示OX-42和Fos阳性细胞的相互关系.结果:在KA致大鼠癫痫发作早期(从15 min到360 min),前脑的小胶质细胞OX-42表达阳性,随着存活时间的变化,OX-42的阳性反应经历逐渐升高又降低的过程;反应强弱:120 min组>360 min组>60 min组>30 min组>15 min组.Fos蛋白在神经元和小胶质细胞中均表达阳性,也呈现逐渐升高又降低的变化,但Fos阳性小胶质细胞出现高峰的时间早于Fos阳性神经元,各组间Fos阳性神经元数量差异均有统计学意义(P<0.01).OX-42阳性小胶质细胞和Fos阳性神经元在前脑主要分布于大脑皮层、海马、杏仁核等部位,两者的分布特征基本一致.结论:小胶质细胞可能和神经元一起参与了KA所致癫痫发作早期的变化.

AIM： To observe the early change of microglia and its relationship with neuron in forebmin of rats with kainic acid （KA）-induced seizure. RESULTS： By means of the singlelabeled immunohistochemical staining method, the expression of OX-42 or Fos was observed in forbmin to show the time course. And the double-labeled immunohistochemical staining for OX-42 and Fos was used to show the relation of OX-42 positive cells and Fos positive cells. RESULTS： The expression of OX-42 in the early stage（ from 15 min to 360 min） of seizure was positive ; OX-42 immunoreactivity was first increased, then decreased and finally disappeared（ 120 min 〉360 min 〉60 min 〉30 min 〉 15 min）. The expression of Fos protein was positive in neuron and microglia; Fos immunoreactivity was first increased, then decreased and finally disappeared, too. But Fos immunoreactive microglia disappeared earlier than Fos immunoreactive neuron, and the number of Fos immunoreactive neuron was statistically significantly different between various groups（ P 〈 0.01 ）. OX-42 immunoreactive microglia and Fos immunoreactive neuron were mostly distributed in cerebral cortex, hippocampus, amygdaloid nucleus, and so on. CONCLUSION： Microglia together with the neuron may both involve the early change of KA-induced seizure.