光动力学疗法联合化疗药物对SHEEC细胞系抑制作用的研究

The study of depressant effect of combinating chemotherapy with PDT by cell line SHEEC

目的:通过光动力联合化疗药物对SHEEC细胞系抑制作用的研究,试图发现有效的联合治疗方案。方法:采用CCK-8检测单一方案(单用顺铂、5-FU、光动力)及联合方案化(先顺铂后光动力、先光动力后顺铂、先5-FU后光动力、先光动力后5-FU)对SHEEC细胞系的抑制率。结果:不同方案均有一定的抑制作用,其抑制率分别为:顺铂(23.61±0.93)%、5-FU(23.44±3.12)%、光动力(22.52±2.83)%、先顺铂后光动力(34.66±2.24)%、先光动力后顺铂(33.74±2.84)%、先5-FU后光动力(33.26±2.74)%、先光动力后5-FU(32.48±2.59)%。结论:单一方案均有一定的抑制作用。联合方案均较单一方案抑制作用强,有统计学差异。联合方案间的抑制作用无统计学差异。

Objective： To study the depressant effect of combinating chemotherapy with Photodynamic therapy by cell line SHEEC. Try to find available combination treatment project. Methods： Detect inhibition ratio of cell line SHEEC which were used by single and combinational project by CCK-8. Results： All the projects had depressant effect, the inhibition ratio： DDP （23. 61 ± 0. 93）% ,5-FU（23.44 ±3.12）% ,PDT（22.52 ±2.83） ,DDP ＋ PDT（34.66 ±2.24）% ,PDT ＋ DDP （33.74 ± 2.84） % ,5-FU ＋ PDT （ 33.26 ± 2.74 ） %, PDT ＋ 5-FU （ 32.48 ± 2.59 ） %. Conclusion ： The single project had depressant effect. Combinational project was more than single project and there were obviously different. Inhibition ratio among the combinational project was not different.