产酸克雷伯氏菌耐氧产氢及其可溶性氢酶耐氧特性研究

Study on Hydrogen Production Activity of Klebsielal oxytoca HP1 and Stability of Its Soluble Hydrogenase under Atmosphere with O_2

目的:考察产酸克雷伯氏菌(Klebsielal oxytoca HP1)耐氧产氢特性及其可溶性氢酶的氧耐受特性。方法:研究K.oxytoca HP1在不同气相氧浓度条件下利用葡萄糖(1%,m/v)、丙酮酸钠(0.5%,m/v)及甲酸(0.1%,v/v)等底物产氢活性的以及K.oxyto-ca HP1可溶性氢酶在空气及氧饱和溶液中催化产氢活性。结果:K.oxytoca HP1在葡萄糖(1%,m/v)底物中具有较高耐氧产氢活性,6h内在气相氧浓度为5%、10%和21%条件下的氢产量分别为厌氧条件下的20.9%、13.7%、8.3%;K.oxytoca HP1可溶性氢酶在空气中孵育12h后,其活性残余85.4%,在氧饱和溶液中活性损失一半约3h。结论:试验结果提示K.oxytoca HP1具有耐氧产氢特性,其可溶性氢酶具有较高氧耐受性,在氢能源的开发中具有潜在的应用前景。

Objective： To study the hydrogen production activity of K. oxytoca HP1 under condition with oxygen stress and the oxygen tolerance ability of its soluble hydrogenase. Method： The hydrogen production activity of K. oxytoca HP1 incubated in glucose （ 1%, m/v）, sodittm pyru- rate （0.5% ,m/v）or formate （0.1%,v/v） under atmosphere with 0%,5%,10% and 21% 02 were studied on, and the remain activity of its soluble hydrogenase incubated in air or pure O2 during 0 - 12h were studied on. Result： The results show that K. oxytoca HPI has high hydrogen production activity in glucose （1%, m/v） substrate under atmospherewith oxygen. Compared to in anaerobic condition, the hydrogen production of K. oxytoca HP1 under atmosphere with 5%, 10% and 21% oxygen was 20.9% ,13.7% and 8.3% respectively in 6h. The soluble hydrogenase from K. axytoca HP1 exhibits considerable oxygen toleration ability, the remain activity was 85.4% after the hydrogenase exposure to air 12h and its activity lost half after the hydrogenase exposed to oxygen 3h. Conclusion： These results suggest that K. oxytoca HP1 and its soluble hydrogenase have potential to .be applied in hydrogen production and application.