黄芩甙对人肝癌BEL-7402细胞增殖和侵袭转移的影响及机制

EFFECT AND MECHANISM OF BAICALIN ON PROLIFERATION,INVASION AND METASTASIS OF HUMAN HEPATOCELLULAR LINE BEL-7402 IN VITRO

目的探讨黄芩甙对人肝癌BEL-7402细胞系增殖、侵袭转移的影响及其机制。方法应用细胞培养技术培养人肝癌BEL-7402细胞,MTT实验、软琼脂克隆形成实验检测黄芩甙对肝癌细胞增殖的影响。通过Boyden小室模型测定其侵袭力,细胞迁移实验测定细胞运动能力,同时观察细胞形态。流式细胞术测定肝癌细胞MMP2、TIMP2表达,免疫组化测定VEGF表达。结果黄芩甙能明显抑制肝癌细胞增殖,细胞侵袭力及运动能力明显下降,且呈量效关系(P<0.05)。形态学观察发现,黄芩甙处理组细胞形态较圆,伪足数目较少;MMP2阳性表达细胞减少,TIMP2阳性表达细胞增多,MMP2/TIMP2比值下降;VEGF表达减少。结论黄芩甙能抑制肝癌BEL-7402增殖、侵袭与转移,其机制可能与直接抑制细胞迁移运动,抑制细胞基质溶解相关基因蛋白MMP2表达,促进TIMP2表达;VEGF表达减少有关。

Objective To study the effect and mechanism of baicalin on proliferation, invasion and metastasis of human hepatocellular line BEL-7402. Methods Human hepatocellular line BEL-7402 was cultured in vitro. MTT and the soft agar colony experiment were used to evaluate cell growth. Invasion assay was conducted in the Boyden chamber, while cell migration and morphology was also observed. The expression of MMP2 and TIMP2 proteins of human hepatocellular line EEL-7402 was determined by flow cytometry. The expression of VEGF was determined by immunohistochemical techniques. Results Baiclin inhibited proliferation, invasion and migration of hepatocellular EEL-7402. Morphologically, the cells treated with baicalin were round with relatively fewer pseudopods. Meanwhile, MMP2 expression was decreased and TIMP2 expression was increased significantly, so MMP2/TIMP2 ratio was significantly decreased. VEGF expression was also decreased obviously. Conclusion Baicalin can inhibit the proliferation, invasion and metastasis of human hepatocellular line BEL-7402, which may be the results of decease in MMP2/TIMP2 ratio and VEGF expression.