MAPK和JAK-STAT途径中酪氨酸蛋白激酶在肝癌组织中的表达及意义

EXPRESSION AND SIGNIFICANCE OF TYROSINE PROTEIN KINASE IN MAPK AND JAK-STAT PATHWAYS IN HEPATOCELLULAR CARCINOMA TISSUE

目的观察MAPK途径和JAK-STAT途径中重要酪氨酸蛋白激酶ERK、P38、C-Jun、JAK、STAT3、STAT5在肝癌组织中的表达及其相互关系,探讨蛋白激酶表达与肝癌患者临床病理特征之间的关系。方法收集原发性肝癌手术切除标本30例,制作组织芯片,酪氨酸蛋白激酶在不同组织中的表达检测采用免疫组化SP法。结果ERK、P38、C-Jun、JAK、STAT3、STAT5在肝癌组织中的表达平均光密度值显著高于肝硬化组(P<0.01)。ERK与C-Jun、JAK、STAT3、STAT5在肝癌组织中的表达呈显著正相关,与P38呈显著负相关。JAK的过度表达与肝癌组织的分化程度有关,在低分化肝癌组织中表达率显著高于高中分化肝癌组织。结论MAPK和JAK-STAT通路的过度活化在肝癌发生发展过程中起重要作用,细胞信号转导系统失去正常的协调平衡可能是导致肿瘤发生的重要原因。

Objective To study the expression and correlation of tyrosine protein kinases in hepatocellular carcinoma tissues, and to observe the correlation between tyrosine protein kinases and clinical features. Methods In 30 cases of HCC, tyrosine protein kinases were detected by immunohistochemistry using tissue chip technology. Results The expressions of ERK, P38, C-Jun, JAK, STAT3 and STAT5 in HCC were significantly higher than in cirrhosis liver tissues （P〈0. 01） . There were significantly positive correlations of ERK with C-Jun, JAK, STAT3 and STAT5; and P38 was negatively correlated with ERK in the HCC tissues. JAK expression showed a significant correlation with turnout differentiation, significantly higher in stage Ⅲ than in stages Ⅰ and Ⅱ Conclusion The excessive activation of the MAPK and JAK-STAT signaling transduction pathway is significant in hepatocellular carcinogenesis, and the unbalance of the signaling transduction pathway may lead to the oncogenesis.