聚焦超声对香猪皮肤重复损伤的组织病理学变化的研究

Histopathological change of the fragrant pig skin induceced by focus ultrasound repetition irradiation

目的:探讨聚焦超声对香猪皮肤重复损伤的组织病理学变化,为临床重复治疗带状疱疹后遗神经痛(Postherpetic neuralgia,PHN)的安全性和有效性提供实验依据。方法:功率2W,频率10.06MHz的参数下对香猪皮肤进行不重复连续直线辐照,隔日相同的条件下再次辐照,共3次,光电镜下观察皮肤变化的情况。结果:肉眼可见皮肤充血水肿明显;光镜下可见皮肤真皮层充血水肿,毛细血管扩张增生,炎症细胞侵润,均未见凝固性坏死;电镜下血管内皮细胞线粒体肿胀,微吞饮小泡数量多,成纤维细胞线粒体肿胀,粗面内质网扩张,神经纤维内线粒体肿胀。结论:(1)此剂量下的超声可对皮肤真皮层造成可逆性损伤。(2)一定剂量一定时间间隔的重复辐照不会引起皮肤的积累损伤,说明超声用于PHN的重复治疗是安全的。

Objective：To explore the histopathological change of the fragrant pig skin induced by focus ultrasound repetition irradiation to offer experiment basis for the safety and effectivessness of clinically treating postberpetic neuralgia（PHN） by repetition irradiation. Methods：The pig skin was irradiated once every other day,altogether three,with irradiation power as 2W, and frequency as 10.06MHz,The skin change was observed under light microscope and electron microscope. Results： Hyperemia and edema in irradiation area after irradiation were observed instantly to the eye;Under light microscope there were hyperemia and edema in epidermis,angiotelectasis and inflammatory infiltrating,and find coagulative necrosis in slice was not observed.Under electron microscope,instantly after ultrasound irradiation every other day,endothelial cell of blood vessel can be found mitochondria swelling and more micropinocytotic vesicles,mitochondria swelling and rough endoplasmic reticulum dilatation were noted for in fibroblasts,and mitochondfia swelling for nerve fibre. Conclusions：（1 ）There is reversibile lesion in epidermis under this dose of ultrasound.（2）Skins don＇t accumulate lesion every other day after irradiation repeatly .which illustrates that it is effective and safe to treat postherpetic neuralgia by ultrasound irradiation repeatly.