沙棘原花青素治疗慢性胃炎的机理及与细胞凋亡的相关性研究

Observe the possible mechanisims of sea buckthorn procyanidins(SBPC) Treat gastritis and study the relationship with apoptosis

目的:观察沙棘原花青素(Sea buckthorn procyanidins,SBPC)对幽门螺杆菌(Helicobacter pylori,Hp)相关性胃炎胃粘膜血小板活化因子(Platelet activating factor,PAF)和Midkine基因(MK)表达的变化,并分析其可能的作用机制。方法:80例幽门螺杆菌感染慢性胃炎患者随机分为2组,治疗组42例给予沙棘原花青素治疗,对照组38例给予西药三联药物治疗。应用胃镜下活检胃黏膜组织快速尿激酶和Warthin-Starry染色法检测幽门螺杆菌,应用免疫组化,Western blotting检测Midkine,血小板活化因子表达的变化。应用末端转移酶介导的dUTP切口末段标记法(TUNEL)检测凋亡小体。第4周复查胃镜。结果:治疗组总有效率为88.1%高于对照组78.9%(P<0.05)。同时,治疗组及对照组治疗后凋亡指数均有明显的下降,组间有显著性差异(P<0.05)。免疫组化,Western blotting检测显示:沙棘原花青素可提高MK和下调PAF的表达,而对照组对胃黏膜MK和PAF的表达影响不大。结论:沙棘原花青素治疗本病机理可能与杀灭HP和提高MK和下调PAF的表达来抑制细胞凋亡有关。

Objective：To observe the effects of sea buckthorn procyanidins（SBPC）on MK and platelet activating factor（ PAF ）in helicobacterpylori-associated gastritis and study the potentional mechanisim. Methods：80 cases were randomized into two groups. 42 cases in the SBPC group which were given SBPC and 38 cases in the control group which were given three combined mecidine. TUNEL technique and immunohistochemistry were used to detect the apoptotic cells. Detection on Biospy Specimens of gastric mucosa was done.H.pylori was detected by urease test and Warthin-Starry method and To observe the effects of sea buekthorn procyanidins （SBPC）on the expressions of MK and platelet activating factor （PAF）,whieh were observed by Immunohischemical and Western blotting method.All of the patients underwent gastroscopy at the 6^th week. Results：The difference of apoptotic index between SBPC group and control group had great statistical significance,with the total efficience of SBPC higher than that of control group（P〈0.05）,The Immunohischemical and Western blotting method showed that SBPC could reduce the gastic mucosa,s expression of PAF,elevate the expression of MK,but the Control Groups hadn＇t this kind of action. Conclusion：The results implied that the possible mechanisims of SBPC are to kill HP,reduce the gastic mucosa,s expression of PAF,and elevate the expression of MK to suppress apoptosis.