慢性低氧环境下大鼠肾组织Apelin-APJ系统的变化及意义

Changes and Significances of Apelin and its Receptor in Kidney Tissue of Rats Chronically Exposed to Hypoxia

目的研究慢性低氧对大鼠肾组织apelin及其受体(APJ)的影响及意义。方法清洁级雄性SD大鼠20只,随机分为正常对照组和低氧组。测定血肌酐(Scr),尿素氮(BUN)及肾组织羟脯氨酸含量,放免法检测肾组织aplein-36的含量,RT-PCR法检测肾组织apelin-36及APJ mRNA的表达。结果①低氧组肾组织羟脯氨酸含量比对照组高37.31%(P<0.01),而两组间的Scr及BUN无显著性差异。②肾apelin-36及APJ mRNA的表达,低氧组均比对照组高(P均<0.05)。③肾组织aplein-36蛋白含量,低氧组比对照组高(P<0.01)。结论Apelin-APJ系统的改变可能与大鼠慢性低氧性肾间质纤维化的进程有关。

Objective To study the expression changes of apelin and its receptor APJ at kidney tissue of rats chronically exposed to hypoxia. Methods Twenty male Sprague-Dawley rats were randomly divided into control group and 4-week hypoxia group. The levels of blood urea nitrogen （BUN）, serum creatinine （Scr） and renal tissue hydroxyproline were measured. The protein level of apelin in the kidney was measured by radioimmunoassay. The mRNA expressions of apelin and APJ in kidney tissue were determined by reverse transcription polymerase chain reaction. Results （1） The content ofhydroxyproline in hypoxia group was 37.3 1%（P〈0.01） higher than that in control group ,while there were no significant differences of Scr and BUN between the two groups. （2） The expressions of apelin and APJ mRNA in kidney tissue of hypoxia group were higher than those of control group （P〈0.0）. （3） The protein level of apelin in kidney of hypoxia rats also was obviously higher than that of control ones. Conclusion The increased protein and gene expressions of apelin and APJ in kidney tissue of rats chronically exposed to hypoxia might be an important factor in the development of renal interstitial fibrosis.