多肿瘤标志物蛋白芯片检测系统在肺癌诊断中的应用价值

Diagnostic value of multiple tumor marker protein biochip detective system for lung cancer

目的:探讨多肿瘤标志物蛋白芯片检测系统对肺癌的诊断价值。方法:用该系统测定128例肺癌患者,26例肺良性病变患者血清中12种肿瘤标志物(CA199,NSE,CEA,CA242,CA125,CA153,AFP,ferritin,free-PSA,PSA,β-HCG及HGH)的水平。结果:肺癌组的芯片阳性率为82%(105/128),显著高于肺良性病组(15.38%,4/26)(P<0.01);肺癌不同分期组间阳性率存在显著性差异,以IV期肺癌组阳性率最高为79.28%(P<0.01),不同分期之间CA199、CEA以及CA242血清水平存在显著性差异(P<0.01);不同病理类型肺癌组间阳性率无显著性差异(P>0.05);CEA阳性率以腺癌组最高,但与其他组织学类型肺癌比较无显著差异(P>0.05);NSE阳性率以小细胞肺癌组最高(P<0.01);单项肿瘤标志物检测与多项肿瘤标志物蛋白芯片联合检测肺癌的阳性率有明显的差异(P<0.01)。结论:多肿瘤标志物蛋白芯片的应用对肺癌的诊断及分期、病理类型及判断预后有一定的临床参考价值。

Objective： To evaluate the diagnostic values of multiple tumor marker protein biochip detective system for lung cancer. Methods： The serum levels of 12 tumor markers,including CA199, NSE, CEA, CA242, CA125, CA153, AFP, ferritin, free -PSA, PSA, -HCG and HCG, were measured in 128 lung cancer patients, 26 patients with benign pulmonary lesion by the detective system. Results： The positive rates were 82% （ 105/128 ）, 15.38% （4/26） in lung concer, benign pulmonary lesion groups, respectively. The lung concer group had significantly higher positive rate than that of the controls（ P 〈 0.01 ） ; There was significant difference of positive rate in various clinical stages of lung cancer （P 〈 0. 01 ） , but not in different pathologic classification. SerumCA199, CEA and CA242 levels were closely correlated with clinical staging（ P 〈0.01 ）. The positive rate of CEA in adenoearcinoma was higher, but no significant difference was observed （ P 〉 0.05 ） ; NSE in small cell lung cancer had the highest positive rate （ P 〈 0.01 ） ; It is statistical significance between the positive rate of one tumor marker detective system and C - 12 multiple tumor marker protein chip detective system for lung eoncer（ P 〈 0. 01 ）. Conclusion ： The application of C - 12 multiple tumor protein chip measurement in diagnosis of lung cancer was helpful.