摘要
目的观察癌热宁栓剂的解热机制。方法采用2,4-二硝基酚致家兔发热模型,测定给药后不同时间的体温变化,并采用双抗体夹心酶联免疫吸附试验(ELISA)检测血清TNF-α、IL-1β含量的变化,使用免疫组织化学方法分析下丘脑蛋白激酶A(PKA)与体温变化关系。结果癌热宁栓剂高、中、低剂量组对家兔非感染性发热有明显的解热作用(P<0.05),其中高、中剂量组与模型组相比差异具有统计学意义(P<0.01)。能够明显降低血清TNF-α、IL-1β含量。相关分析显示,5组家兔的体温变化分别与下丘脑PKA含量变化呈正相关。结论癌热宁栓剂对由2,4-二硝基酚引起的非感染性发热动物模型具有解热作用。能够抑制血清中TNF-α、IL-1β含量的增多,同时,降低下丘脑cAMP-PKA的水平,可能是癌热宁栓剂的重要解热机制之一。
Objective To adverse reaction of Cancer Rening suppository in the treatment of fever cancer. Methods The models of noninfected fever induced by 2,4-binitro-phenol was used to observe the changes of temperature in different time, TNF-α and IL-1β in the serum of rabbit were determined by double antibodies sandwich Enzymelinked Immurosorbent Assay. Immuno-histochemical assay was used to detect the expression between PKA activities of hypothalamus and the changes of temperature. Results Different doses of Cancer Rening suppository （13. 5 g/kg, 4. 5 g/kg, 1.5 g/kg） reduced the febrile responses of rabbit induced by noninfected fever. In the febrifuge experiment of rabbit, both the high and middle group have the febrifuge effect obviously. Changes of TNF-α,IL-1β in serum and PKA in hypothalamus during noninfected fever induced by 2,4-binitro-phenol in rabbits are significantly increased. Cancer Rening suppository has a protective effect on noninfected fever induced by 2,4-binitro-phenol in rabbits, decreasing the expression of TNF-α, IL-1β in serum and PKA in hypothalamus obviously. Correlation analysis indicated that the changes of the rabbit body temperature were positively correlated with the contents change of PKA in the hypothalamus of the five rabbit groups. Conclusion The antipyretic mechanisms of Cancer Rening suppository may be related to its decreasing the contents of TNF-α, IL-1β in serum and PKA in hypothalamus. It may be an important antipyretic mechanism of Cancer Rening suppository.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第8期543-546,共4页
Cancer Research on Prevention and Treatment
基金
天津市卫生局中医
中西医结合科研课题资助项目(03043)
