STAT3信号转导通路及其靶基因产物与卵巢癌恶性潜能的关系

Constitutive Activation of STAT3 Pathway and Overexpression of Target Gene Products Correlate with Malignant Potential in Human Ovarian Carcinoma

目的分析STAT3及其靶基因产物Cyclin D1与Caspase-3在卵巢癌组织中的表达情况,探讨STAT3及其靶基因产物在卵巢癌发病机制中的作用。方法收集经手术切除的卵巢癌标本40例,应用Western blot检测40例卵巢癌组织、正常卵巢组织中STAT3及其靶基因产物Cyclin D1与Caspase-3表达。结果p-STAT3、Cyclin D1与Caspase-3表达水平在卵巢癌组织中明显高于正常组织(肿瘤vs正常,P=0.028,0.035,0.047);p-STAT3与Cyclin D1表达水平与FIGO分期相关(P=0.026,P=0.041)。p-STAT3及Cyclin D1在卵巢癌组织中表达情况呈线性相关(r=0.412,P<0.05)。结论STAT3信号转导通路可能在卵巢癌发生过程中起重要作用,检测卵巢癌中STAT3及其靶基因产物的表达可以反映肿瘤的恶性潜能。

Objective The purpose of this study was conducted to investigate the expression of Signal transducer and activator of transcription 3 （STAT3） and target gene products including Cyclin D1 and Caspase-3 in human ovarian carcinoma tissues, and to explore the mechanisms in tumorigenesis of ovarian carcinoma. Methods Primary ovarian cancers and normal ovarian tissue were obtained from 40 patients undergoing ovarian cancer resection. Western blot analysis was used to measure the expression of STAT3, p-STAT3, Cyclin D1, and Caspase-3 in cancerous tissues, normal ovarian tissues. Results p- STAT3, Cyclin D1, and Caspase-3 protein levels were increased in ovarian cancer compared with adjacent normal tissue （P = 0. 028,0. 035,0. 047）. Overexpression of p-STAT3 and Cyclin D1 were correlated with FIG（） stage in ovarian cancer （P= 0. 026,0. 041）. It was found that Cyclin D1 was in a positive line ar correlation fashion with p-STAT3 in tumor （r= 0. 412,P〈0. 05）. Conclusion These findings provide evidence that STAT3 signaling pathway may play an important role in the tumorigenesis of ovarian carcinoma, and detecting the expression of STAT3 and its target gene products may predict the malignant potential of ovarian carcinoma.