一种脱细胞真皮基质制备方法的研究

Research to a preparation method of acellular dermal matrix

目的:研究脱细胞真皮基质(acellular dermal matrix,ADM)的一种新的制备方法。方法:取大鼠背部皮肤制成厚度0.8mm的脱毛皮片,置入1mol/L NaCl溶液中24h,揭去表皮层,再浸入0.5%Triton X-100溶液中室温震荡48h,得到ADM。取标本行组织学观察,并行异体移植实验。结果:NaCl-Triton法制作的ADM,细胞成分脱除彻底,胶原纤维排列规则,没有明显的免疫原性,生物相容性好。结论:采用NaCl-Triton法制备ADM,价廉而有效,利于推广应用。

Objective To research a new method of producing acellular dermal matrix （ADM）. Methods The non-hair skin grafts with 0.8mm in thickness were acquired from the back of Wistar rats＇. Then the grafts were treated with 1M NaCl 4℃ for about 24 hours to rip away the cuticular layers, and the remained dermis were immerged into 0.5%Triton X-100 shaking in normal temperature for about 48 hours to obtain ADM. Took samples of the ADM to do histology observation, and homoplastic tronsplantation. Results There was no cell constituent remained in ADM produced by NaCl-Triton method, and the collagen fibers were in a regular arrangement. ADM had very low immunocompetence and good biocompatibility. Conclusion Using NaCl-Triton method to produce ADM was low-cost and effective, so it was advantageous to populize application.