微创大鼠体外循环模型的建立

A minimally invasive model of cardiopulmonary bypass in rats

目的:建立微创大鼠体外循环模型,为研究体外循环术后病理生理变化和评价治疗策略提供可靠、经济的实验平台。方法:20只成年SD大鼠,采用颈静脉、尾动脉、股动脉插管建立体外循环通路,在100 ml/(kg.min)流量下维持转流60 min,监测心率、血压、血气及电解质结果分析。结果:20只大鼠全部穿刺成功,在设定条件下完成体外循环,术后存活均>1周。结论:该模型具有经济、简单、微创、体外循环参数设置接近临床、模型成功率高、术后生存期长等优点,可作为研究体外循环后病理生理变化和评价治疗策略的可靠工具。

Objective： To develop a minimally invasive model of eardiopulmonary bypass （ CPB ） in rats. Methods： Twenty adult SD rats were anesthetized, and the external jugular vein, the tail artery and the femoral artery were cannulated to establish CPB, which maintained at the flow rate of 100 mL/（ kg · min） for 60 min, while the heart rate, blood pressure, blood gas and electrolytes were monitored and analyzed. Results： All the animals successfully underwent cannulation and CPB and survived more than 1 week after the procedure. Conclusion： The CPB procedure, minimally invasive, easy to perform and with a high rate of survival, can be used as an effective tool for the study of the pathophysiologic changes and the evaluation of the therapeutic strategies associated with CPB.