瘦素对小鼠成骨细胞MC3T3-E1增殖及OPG/RANKL mRNA表达的影响

Leptin effect on the proliferation and OPG/RANKL mRNA expression in MC3T3-E1 osteoblasts of mice

目的初步探讨瘦素对小鼠成骨细胞MC3T3-E1细胞增殖及骨保护蛋白、核因子κB激活受体配体mRNA表达的影响。方法以小鼠成骨细胞MC3T3-E1为体外实验模型,用MTT法检测瘦素作用MC3T3-E1细胞后的增殖情况,RT-PCR方法检测OPG、RANKL mRNA表达。结果瘦素(10、20、40、80和160ng.mL-1)作用于MC3T3-E1细胞24h后,可促进其增殖,OD值显著增加(P<0.01),增加骨保护蛋白mRNA表达,同时降低核因子κB激活受体配体mRNA表达,且呈浓度依赖性。结论瘦素促进MC3T3-E1细胞增殖,同时通过调节骨保护蛋白mRNA和核因子κB激活受体配体mRNA的表达,从而促进骨形成,抑制骨吸收。

Objective To study the effect of leptin on proliferation and the expression of osteoprotegerin （OPG） and receptor activator of NF-κB ligand （RANKL） mRNA in MC3T3-E1 osteoblasts of mice. Methods The osteoblasts MC3T3-E1 were cultured in vitro, the effects of different levels of leptin on osteoblast proliferation were assessed by MTT colorimetry; the expression OPG and RANKL mRNA in MC3T3-E1 was analyzed by semiquantative RT-PCR. Results Proliferation of MC3T3-E1 was promoted by leptin（ 10,20,40,80,160 ng·mL^-1 ） after 24 hour, Semi-quantative RT-PCR examination revealed： leptin promote OPG mRNA expression and inhibit the RANKL mRNA expression, in concentration-dependent manner. Conclusion Leptin promotes proliferation in MC3T3-E1 osteoblasts of mice, and promotes OPG mRNA expression and inhibits RANKL mRNA expression, accordingly promotes bone formation, and inhibits bone resorption.