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乙肝病毒感染导致Mrell下调及基因组断裂 被引量:3

HBV infection downregulated Mre11 expression and induced genome instability
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摘要 【目的】乙肝病毒的持续感染与肝细胞癌的发生密切相关。本文探讨了乙肝病毒感染后导致宿主蛋白Mre11的变化,并研究这种蛋白的变化可能导致肝癌发生的机制。【方法】本文通过Western blot检测了乙肝病毒感染HL7702细胞及肝癌组织标本的Mre11蛋白的变化,并且通过RNA干涉的方法干扰了Mre11的表达,用连接介导PCR检测基因组的变化。【结果】本研究发现乙肝病毒感染细胞导致Mre11表达下调,肝癌组织也可以发现Mre11表达下调;下调Mre11表达可以导致细胞基因组的断裂增多。【结论】HBV感染导致Mre11表达下调导致基因组不稳定,这可能与HBV感染导致细胞恶性转化相关。 [Objective] Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mre 11 fluctuations after HBV infection which might be eventually contributed to cell transformation. [Methods] Western Blot was monitored to detect the expression of Mrel 1, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks, [Results] Mrel 1 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mrel 1 knockdown caused DNA instability. [Conclusion] Mrell expression downregulation contributed at least partially to cell transformation caused by HBV infection.
出处 《微生物学报》 CAS CSCD 北大核心 2008年第8期1031-1034,共4页 Acta Microbiologica Sinica
基金 国家自然科学基金面上项目(30772605 30700413) 武警总医院资助项目(WZ200515)~~
关键词 乙肝病毒 肝细胞癌 Mre11 DNA损伤修复 Hepatitis B virus hepatocellular carcinoma Mrell DNA damage repair
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