曲格列酮增加Hela细胞的自噬和混合型细胞死亡

Stimulating autophagosome formation and inducing mixed cell death in HeLa cells by Troglitazone

【目的】为了研究Troglitazone(Trog)对HeLa细胞自噬和程序性细胞死亡的影响。【方法】利用电镜、荧光显微镜、免疫杂交、流式细胞计数等对经Trog处理后的HeLa细胞进行了细胞发生自噬和死亡情况的观察。【结果】电镜、荧光显微镜的结果均提示,Trog能够诱导HeLa细胞自噬活动的增加;免疫杂交显示,该药物能增加自噬相关基因LC3的表达,并于刺激后4h达到高峰;除了能使细胞凋亡外,Trog也可以造成细胞的坏死。【结论】上述结果表明,曲格列酮可以引起混合型细胞死亡。

[Objective] To study the effect of troglitazone [Methods] Fluorescence microscopy, electron microscopy, on autophagy and programmed cell death of HeLa cells. western-blotting and flow cytometry were used. [Results] Troglitazone increased the autophagosome formation of HeLa cell as observed by fluorescence and electron microscopy. Immunoblotting further confirmed cell morphology observation upon the reagent stimulating by detecting an enhanced expression of LC3, an autophagic related gene. Besides apoptosis, troglitazone triggered a necrotic cell death in flow cytometry analysis. [Conclusion] Troglitazone induced mixed type of cell death in HeLa cells.