小鼠放射性肠炎模型的建立

Comparison of radiation enteritis mouse models established at different doses

目的 建立小鼠放射性肠炎模型。方法 雌性BALB／c小鼠84只，随机分为对照组（24只）、6Gy组（28只）及10Gy组（32只），^66Co γ射线对小鼠腹部一次性照射，在照射后3、7、14和21d检测血象及肝、肾功能，并取肠道组织做病理及一氧化氮（NO）检测。结果 照射后3、7d，6Gy及10Gv组WBC、PLT均较对照组降低；10Gy组肝功能异常；10Gy组照射后3d肠道组织NO水平降低；照射后3、7d，2个照射组小肠绒毛高度降低．隐窝深度变浅、数目减少，隐窝腔变大，均出现放射性肠炎改变，10Gv组死亡较多，6Gy组更能模拟临床放射性肠炎，为研究提供模型。结论。^60Co射线一次性腹部照射建立小鼠放射性肠炎模型，6Gy照射剂量更为合适。

Objective To explore the optimum dose for the mouse radiation enteritis model by abdominal irradiation（ AR）. Methods 84 BALB/c female mice were randomly divided into three groups as control group, 6Gy group and 10 Gy group. For each group, mice were sacrificed at 3, 7, 14 and 21 days after single abdominal irradiation of Cobalt-60, respectively. The blood was sampled to detect the hemogram, the liver and renal function. 5 cm sections of the terminal ileum were fixed in formalin for histopathological examination including the mean height of villous and the number of crypt. Small intestinal mucosal samples were homogenized for NO concentration analysis. Results In 10 Gy group, WBC and PLT decreased obviously, and the liver function was damaged. The length of villi and the number of crypt were significantly reduced in irradiated groups compared with control group, especially in 10 Gy group. Intestinal NO concentration decreased in 10 Gy group in 3 d. Conclusions To establish the mouse model of irradiated enteritis single 6 Gy ^60 Co abdorminal irradiation is optimum.