针刺对去卵巢大鼠骨质疏松症骨代谢及血清雌二醇含量的影响

Effects of Acupuncture on Bone Metabolism and Serum Estradiol Level in Ovariectomy-induced Osteoporosis Rats

目的:研究针刺对去卵巢大鼠骨代谢生化指标及雌二醇的影响,探讨运用针刺方法对骨质疏松症模型进行干预治疗的机制。方法:采用6月龄雌性SD大鼠40只,随机分为假手术组、模型组、针刺组和己烯雌酚对照组,每组10只。除假手术组外,其余各组切除双侧卵巢建立骨质疏松症模型。造模2月后开始治疗,假手术组、模型组大鼠用生理盐水灌胃,己烯雌酚组以己烯雌酚生理盐水混悬液(2.25μg/ml)灌胃,均以1ml/100g的标准灌服,每日1次,连续灌服2月;针刺组针刺双侧"大杼""肾俞""脾俞"穴,每日1次,每次持续30min,每隔10min轻捻转行针1次,10次为1个疗程,共治疗6个疗程。用常规生化法检测血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)水平,用放射免疫法检测血清骨钙素(BGP)、雌二醇(E2)水平。结果:与假手术组比较,模型组子宫湿重、E2水平均显著降低(P<0.01),ALP、BGP、TRAP水平均显著升高(P<0.01,0.05);与模型组比较,针刺组及己烯雌酚组能明显抑制子宫萎缩(P<0.01),血清E2显著增高(P<0.01),ALP、BGP及TRAP水平显著降低(P<0.01),体重显著降低(P<0.01);针刺组与假手术组比较,ALP、BGP及TRAP水平差异无显著性意义(P>0.05),两组体重增长水平相当(P>0.05)。结论:针刺能明显阻止去卵巢大鼠体重的增加,提高血清E2水平,显著控制ALP、BGP与TRAP的增高,可能对骨质疏松症有一定的防治作用。

Objective To observe the effect of acupuncture（Acu） on bone metabolism and serum estradiol （E2） in ovariectomized （OVX） rats for studying its underlying mechanism in treating osteoporosis. Methods Forty female SD rats of six months were randomized into sham operation （sham）, model, Acu and Diethylstibestrol （DES） groups, with 10 cases in each. Postmenopausal osteoporosis model was established by removing ovaries under anesthesia. In Acu group, bilateral ＂Dazhu＂ （BL 11）, ＂Shenshu＂ （BL 23） and ＂Pishu＂ （BL 20） were punctured and stimulated for 30 minutes, once daily for 60 days. Rats of DES group were drenched with saline＋ DES （22.5 μg/ml） 1 ml/100 g, once daily for 60 days. At the end of experiments, blood samples were collected from femoral artery for assaying serum alkaline phosphatase （ALP）, tartrate-resistant acid phosphatase （TRAP） contents by using biochemistry, and serum bone gla protein （BGP） and E2 levels by immunoradioassay. Results Compared with sham group, uterus wet weight, serum E2 content in model group decreased significantly （P 〈 0.01 ）; while body weight, serum ALP, BGP and TRAP levels in model group increased significantly （P〈0.01, 0.05）. Compared with model group, uterus wet weight and serum E2 content in Acu and DES groups increased significantly （P〈0. 01） ; while body weight, serum ALP, BGP and TRAP levels decreased considerably （P〈0. 01）. No significant differences were found between Acu and DES groups in serum E2, ALP, BGP and TRAP levels （P〉 0.05）. Conclusion Acupuncture can suppress abnormal increase of body weight and decrease of serum E2 level, and significantly downregulate serum ALP, BGP and TRAP levels in OVX rats, which may contribute to its effect in relieving osteoporosis.