青藤碱在家兔体内的药代动力学研究

Pharmacokinetics of sinomenine in rabbits

目的:建立高效液相色谱法测定青藤(Sinomenine)碱在家兔体内的血药浓度并研究其药动学。方法:6只家兔分别给予青藤碱45mg/kg灌胃,于给药后0.25h、0.5h、1.0h、1.5h、2.0h、3.0h、4.0h、6.0h、8.0h、10.0h、12.0h分别取血2ml,采用高效液相色谱法检测血药浓度,3P97软件处理数据并进行药代动力学研究。结果:T1/(2Ka)为(0.31±0.26)h,T1/(2Ke)为(3.15±0.82)h,Tmax为(1.05±0.53)h,Cmax为(15.09±4.15)μg/ml,AUC0→∞为(84.27±13.02)(μg·h)/L,AUC0→T为(78.29±13.46)(μg·h)/L。结论:家兔灌服青藤碱后药物吸收和消除快,达峰时间短,血药浓度高。

Objective：To establish a HPLC method for measurement of serum concentration of sinomemine in rabbits and explore it＇s pharmacokinetics. Methods：6 rabbits were intragastricly administrated sinomenine with the dosage of 45mg/kg,2 ml blood were collected at 0.25h,0.5h,1.0h,1.5h,2.0h,3.0h,4.0h,6.0h,8.0h,10.0h and 12.0h after oral administration. concentrations of drug were determined by HPLC,datas were calculated with 3P97 software and pharmacokinetics was explored. Results：T1/2（Ka） was（0.31 ± 0.26）h,T1/2（Ke） was（3.15 ± 0.82）h,Tmax was（ 1.05 ± 0.53 ）h,Cmax was（ 15.09 ± 4.15 ） μ g/ml,AUC0→∞ was （ 84.27 ± 13.02 ） （ μ g·h ）/L, AUC0→T was （ 78.29 ± 13.46 ） （ μg·h ）/L. Conclusion ： absorption and elimination of Sinomenine were quickly and Tmax is short,concentration of drug is high in rabbits after intragastric administration.