N-乙酰半胱氨酸对急性肺损伤大鼠的保护作用

The Protective Effect of N-acetylcysteine on Acute Lung Injury in Rats Induced by Oleic Acid

目的:探讨N-乙酰半胱氨酸(NAC)对油酸(OA)诱导的急性肺损伤(ALI)大鼠的保护作用。方法:成年雄性SD大鼠18只,随机分成对照组、损伤组(ALI组)和N-乙酰半胱氨酸组(NAC组),后两组在注射OA后3 h检测肺湿重与肺干重比(W/D),肺组织匀浆髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)及肺组织病理检查。结果:ALI组MPO、MDA明显升高,SOD明显降低,与对照组比较有明显差异(P<0.01),NAC组MPO、MDA低于ALI组,SOD明显升高,P<0.01;ALI组MPO水平与肺组织病理损伤程度成正相关(r=0.793,P<0.01);肺组织病理组织学显示,NAC预处理能明显改善肺组织病理损伤。结论:NAC对ALI大鼠具有保护作用,其保护机制可能与清除氧自由基有关。

Objective： To investigate the protective effect of N-acetylcysteine （NAC） on lung injury in rats induced by oleic acid （OA）. Methods： Eighteen male Sprague-Dawley rats were randomly di- vided into acute lung injury （ALI） group （ n = 6）, N-acetylcysteine （NAC） group （ n = 6 ） and control group （n = 6）, The lung tissues were collected in 3h after OA or saline administration. Lung weight/dry ratio（W/D）, the concentrations of lung homogenate myeloperoxidase （MPO）, malondiadehyde （MDA）, and superoxide dismutase （SOD） were measured. Meanwhile, The pathological changes of lung were examined with light microscope. Results： In ALI group, levels of lung homogenates MPO, MDA were significantly higher and the level of SOD were significantly lower than those in control group and NAC group（All P 〈0.01）. The levels of MPO in rats of ALI group have a significantly positive correlation with lung pathological injury scores （r =0. 793, p 〈 0.01 ）. Histopathologic findings showed less lung injury in NAC group than in ALI group. Conclusions： NAC might have effect of alleviating the lung injury induced by oleic acid , of which the mechanism might be associated with free oxygen radical elimination.