转移抑制基因MKK4在大肠癌中的表达与其生物学行为及预后的关系

Expression of MKK4 in Colorectal Carcinoma and Its Relationship with Biological Behavior and Prognosis of the Tumor

目的:检测有丝分裂素激活蛋白激酶激酶4(Mitogen-activated protein Kinase Kinase 4,MKK4)在大肠癌中的表达与其生物学行为及预后的关系。方法:应用免疫组织化学Envision二步法对58例大肠癌和20例大肠黏膜慢性炎症MKK4表达进行检测。结果:大肠癌组织中MKK4阳性表达率为75.9%,慢性炎症组织中MKK4阳性表达率为100%,大肠癌MKK4阳性表达率较慢性炎症组织低,差异有显著性(P<0.05);大肠癌组织中MKK4阳性表达率与患者年龄、性别、肿瘤部位及大小无关(P>0.05),与有无淋巴结转移、临床分期、浸润深度及生存期有关(P<0.05)。结论:MKK4可能在大肠癌侵袭转移的发展过程中具有重要作用,与患者预后相关。

Objective： To investigate the expression of mitogen-activated protein kinase kinase 4 （MKK4）in colorectal carcinoma and its relationship with the biological behavior and prognosis of the tumor. Methods ： MKK4 expression in tissue specimens of 58 cases of colorectal carcinoma and 20 cases of chronic inflammatory were detected by using immunohistochemistry （IHC） method, and Envision two-step staining method. Results： Positive rate of MKK4 protein in colorectal carcinoma tissues （ 75 9 % ） was lower than that in chronic inflammatory tissues （ 100 % ）. The difference was significant （P 〈0.05 ）. MKK4 expressions in carcinoma tissues correlated with lymph node metastasis （P 〈 0. 05）, Dukes stage（P 〈0.05）, invasive depth （ P 〈0.05）, and survival time（P 〈0.05）, but not with age, gender, location or tumor volume （ P 〉 0.05 ）. Conclusions ： MKK4 may play an important role in the progression , metastasis of human colorectal carcinoma, and closely correlates with patients＇ prognosis.