达肝清对小鼠免疫功能影响的实验研究

Influence of Daganqing on the Immunity in the Mice

目的探讨达肝清对正常小鼠和免疫抑制小鼠免疫功能的影响。方法用达肝清给正常小鼠和环磷酰胺免疫抑制小鼠灌胃,然后用称重法检测小鼠肝脾重量、溶血试验检测血清溶血素含量、QHS法检测抗体形成细胞数、RIA法检测IL-2含量、MTT法检测NK细胞活性和T淋巴细胞增殖功能。结果达肝清对正常小鼠免疫功能无显著影响,对CXT免疫抑制小鼠有显著的抵抗作用,能增加免疫抑制小鼠肝脾的重量、促进抗体生成和IL-2分泌,增加NK细胞活性和T淋巴细胞增殖功能。结论达肝清对正常小鼠的免疫功能无显著影响,但能抵抗CTX对小鼠免疫功能的抑制作用。

OBJECTIVE To investigate the effect of Daganqing on the immunity in the normal or immunosuppressive mice. METHODS Daganqing was oral in normal or immunosuppressive mice and then the weight of immune organs, the numbers of hemolysin, antibody form cells, IL-2, the phagocytic activity of macrophage and the activities of T lymphocyte and NK cell were detected by the methods of weighting method, haematolysis experiment, QHS, RIA, MTT, respectively. RESULTS Daganqing could promote the weight of immune organs and the numbers of hemolysin and antibody form cells, and promote the IL-2 level in the serum, promote phagocytic activity of macrophage, T lymphocyte proliferation and NK cell activity in the immunosuppressive mice but did not have significant effect in the normal mice. CONCLUSION The results suggest that Daganqing has up-regulation on humeral immunity and cells-mediated immunity in the immunosuppressive mice but hasn＇t significant effects in the normal mice.