白藜芦醇对豚鼠心室肌细胞钠电流的影响

Effects of Resveratrol on Sodium Current in Guinea Pig Ventricular Myocytes

目的研究白藜芦醇(resveratrol,RES)对豚鼠心室肌细胞钠电流(INa)的影响,探讨其心肌保护机制。方法用全细胞膜片钳技术记录RES对单个心室肌细胞INa的作用。结果RES(10,30,100μmol.L-1)浓度依赖性的抑制豚鼠心室肌细胞INa,其中30μmol.L-1对INa的抑制率为(17.3±3.1)%(P<0.005),100μmol.L-1抑制率(52.7±10.2)%(P<0.01),抑制作用迅速,用药后3 min左右即开始起效,洗脱后均可以完全恢复;100μmol.L-1使半数最大失活电压(V1/2)由(-89.3±2.0)mV变化到(-100.7±3.3)mV(P<0.005),RES未改变S和半数最大激活电压(V1/2)。结论RES浓度依赖性的抑制INa,作用出现快,并且可逆。

OBJECTIVE To study the effects of resveratrol （RES） on sodium current （INa） in guinea pig ventricular myocytes, and to investigate the mechanism of cardioprotection. METHODS Single cardiac myocytes were isolated by enzyme, and the effect was assessed by whole-cell patch clamp technique. RESULTS RES （ 10,30,100 μmol · L^-1 ） was shown to inhibit INa of guinea pig ventricular myocytes in a concentration dependent manner, and the inhibition ratios of 30,100 μmol · L^-1 were 17.3% ± 3.1% （P 〈 0. 005） and 52.7% ± 10.2% （P 〈 0.01 ）, respectively. RES acted quickly （ about 3 min） and its effects were reversible completely after a 10 min washout. For availability, V1/2 was （ - 89.3 ± 2.0） mV in control and （ - 100.7± 3.3 ） mV in 100 μmol · L^-1 RES （ P 〈 0. 005）. The maximal activating voltage and S of INa were not changed. CONCLUSION RES can exhibit direct inhibitory effects on INa rapidly in guinea pig ventricular myocytes, and these effects are reversible.