气相色谱法测定二十二醇乳膏的含量

Determination of N-docosanol Cream by GC

目的建立二十二醇乳膏的含量测定方法。方法用气相色谱法测定了二十二醇的含量，以二十二烷为内标，色谱柱为SPB-5（15m×0．53mm×1．5μm）毛细管柱，柱温为230℃，气化室温度250℃，检测器温度300℃，分流比1：10。结果回收率100．7％，RSD=1．70％，线性范围为：41．08～410．8μg·mL^-1，线性方程Y=-0．0328＋0．004377X，r=0．9995，重复性RSD=0．85％，n=6。结论方法可靠，可以用于控制二十二醇乳膏的质量。

OBJECTIVE To establish a GC method for the determination of n-docosanol cream. METHODS GC was used to determine rl-docosanol Cream. The seperatiorl was performed on SPB-5 （ 15 m × 0.53 mm × 1.5 μm） capillary column. N-docosarle was added as internal standard. The inject temperature, column temperature and detect temperature was set at 250 ℃, 230 ℃ , 300 ℃, respectively. The splite ratio was 1 ： 10. RESULTS The average recovery was 100.7% , RSD = 1.70%. The calibration curve was linear between 41.08 -410.8 μg · mL^-1 ,r =0. 999 5,with good reproducibility （RSD =0.85% ,n =6）. CONCLUSION The method is accurate and carl be used for the quality control of n-docosanol Cream.