核黄素光化学法灭活单采血小板细菌的效果评价

Effect of riboflavin photosensitizer on bacteria inactivation in APPC

目的探讨核黄素光化学法灭活单采血小板细菌的效果及血小板一系列生化和生理学指标的改变。方法将28ml浓度为500μmol／L核黄素加到250ml的单采血小板悬液中，再将一定浓度的G^＋菌（表皮葡萄球菌ATCC12228）和G^＋菌（大肠埃希菌ATCC25922）分别注入上述混悬液中，经265—370nm广谱紫外光6．2J／ml照射8～10min后，测定其浓度，并检测血小板体外参数的变化情况。结果经浓度50μmol／L的核黄素结合强度为6．2J／ml紫外光照射8—10min，可将一定浓度的模型菌灭活至〈1．22logCFU／ml。结论核黄素结合紫外光照射可以有效灭活细菌，而单采血小板的各项生化、生理学指标和阴性对照比较均差异无显著性。

Objective To explore a photochemical method that can inactivate selected bacteria using riboflavin plus UV light and the inactivation effects of UV light plus riboflavin on bacteria in terms of a series of biochemical and physiology parameters. Methods First, model bacteria （Staphylococcus epidermidis ATCC12228 and Escherichia coli ATCC25922） were added to apheresis platelet （250 ml） ; second 500 μmol/L riboflavin （28 ml） was added to the blood bag; third broadband UV light was used to illuminate the blood bag; last the bacteria titer was measured and the inactivation effects of UV light plus riboflavin on bacteria was detected. Results The new technology reduced the model bacteria from 5.41 logCFU/ml to 〈 1.22 logCFU/ml in 10 minutes. Conclusion Riboflavin plus ultraviolet light could significantly inactivate selected bacteria while adequately maintain PLT cell quality.