卡那霉素在耳蜗毛细胞中的积聚部位

Accumulation sites of kanamycin in the organ of Corti by microautoradiography

为进一步探讨卡那霉素的耳中毒机理，应用放射自显影和电镜技术观察了３Ｈ标记卡那霉素在５只豚鼠耳蜗毛细胞中的积聚部位。发现卡那霉素主要积聚在线粒体、质细胞膜和静纤毛。卡那霉素积聚在线粒体的原因可能是由于线粒体具有与细菌相同的蛋白质合成方式，卡那霉素可以其杀菌的相同方式抑制线粒体的蛋白质合成。卡那霉素积聚在质膜的原因可能由于这里是药物进入细胞的途径。而药物积聚在静纤毛的原因可能与卡那霉素对糖萼的化学亲和力有关。

The accumulation sites of the3Hlabelled kanamycin in the organ of Corti were observed with electron microscope. The results showed that the accumulation of kanamycin was mainly in the mitochondria, cell membrane and stereocilia of the hair cells. The possible mechanism of the kanamycin accumulation in the mitochondria is their similarity with bacteria in the protein synthesis, i.e. the way that kanamycin might inhibit the protein synthesis in the mitochondria is similar to that of bacteria. The kanamycin accumulation in the cell membrane may indicate the entrance of kanamycin into the hair cells. The kanamycin deposition in the stereocilia was most likely related to its high chemical affinity with glycocalyx.