基因重组人表皮生长因子对胃粘膜保护的实验研究

Protection of gastric mucosa from ethanol induced injury by synthetic human epidermal growth factor in rats

为探讨基因重组人表皮生长因子（ｈＥＧＦ）对胃粘膜的保护作用，作者将５４只ＳＤ大鼠，随机分６组给药，禁食，灌无水乙醇。结果：西咪替丁组和对照组均见急性胃扩张，对照组胃液呈血性改变，各组胃溃疡指数分别是：对照组最高为５８，ｈＥＧＦⅠ组５３，ｈＥＧＦⅡ组４６（Ｐ＜０．０５），ｈＥＧＦⅢ组１１（Ｐ＜０．０１），ｈＥＧＦⅣ组１９（Ｐ＜０．０１），西咪替丁组３９（Ｐ＜０．０５）。鼠血ＥＧＦ水平与胃粘膜抗损伤作用相关；同一剂量的ｈＥＧＦ、皮下注射效果优于口服。结论：基因重组的ｈＥＧＦ有明显的抗乙醇损伤的胃粘膜保护作用，其效果在６０～１２０μｇｋｇ－１ｄ－１范围内呈量效关系，重组ｈＥＧＦ很有希望成为治疗人消化性溃疡的一种内源性药物。

The present study was designed to determine whether human epidermal growth factor (hEGF) was protective of gastric mucosa against ethanol induced injury in rats. Fiftyfour SD rats weighing 200250 g that prevented from eating for 24 hour were divided into six groups that received hEGF, cimetidine, 0.9% saline, and absolute ethanol, respectively. The results showed that acute gastric dilatation developed in the control and cimetidine group and bloody gastric juice was found in the control group. The ulcer index in control was 58, in hEGF I was 53, hGEF Ⅱ was 46 (P<0.05), in hEGF Ⅲ was 11 (P<0.05) and in hEGF Ⅳ was 19 (P<0.01), and in cimetidine group was 39 (P<0.05). Conclusions: This study confirmed that hEGF protects the gastric mucosa against ethanol induced damage, its effects was associated with levels of mouse blood epidermal growth factor (EGF) and increased at doses 60120 μg·kg-1·d-1. Another finding was that the factor was more effective with injection than with oral route at the same dose.