深圳地区HIV-1感染者的本底耐药水平调查初探

PRELIMINARY RESEARCH ON BASELINE DATA OF DRUG RESISTANT MUTATIONS AMONG TREATMENTS OF NAIVE HIV INFECTED PERSONS IN SHENZHEN BY GENOTYPING METHOD

[目的]建立一套完善,可靠,适合深圳人群的HIV-1耐药性基因型检测方法,调查分析深圳地区未接受抗病毒治疗的HIV感染者/AIDS患者的病毒株耐药突变及其本底流行情况。[方法]从未接受抗病毒治疗的HIV-1感染者血浆中提取病毒RNA,采用RT-PCR扩增目的基因片断,并对扩增片断进行序列测定和分析。[结果]通过HIV-1耐药性基因型检测法能够获得1300bp长度的目的基因片断。经序列分析所得的21份RT基因结果中有8份对NRTI产生耐药突变(38.5%),5份对NNRTI产生耐药突变(23.81%);另外分析所得的12份PR基因结果中1份产生对蛋白酶抑制剂主要耐药突变(8.33%),5份产生对蛋白酶抑制剂次要耐药突变(41.67%)。[结论]HIV-1耐药性基因型检测法能有效地监测HIV-1感染者血浆中的耐药性病毒株的存在,并且检测结果表明,深圳地区未接受抗病毒治疗的HIV-1感染者中已经存在耐药突变株。

[Objective] To construct a complete and reliable HIV-1 genotyping method which was suited to the HIV positive population in Shenzhen that can be used to analyze the drug resistant mutations and prevalence of HIV-1 strains among them. [Methods] HIV-1 viral RNA samples were collected from plasma samples of HIV-1 infected persons without HAART and were amplified by nested PCR using specific primers; then the amplified fragments were sequenced and analyzed. [ Resuits] The 1300bp fragments of RT-PCR products were obtained from the samples. Among 21 RT genes obtained from sequencing, there were 8 NRTI resistance mutations （38.5%） and 5 NNRTI resistance mutations （23.81%）. In addition, there were 1 PI major resistance mutations （8.33%） and 5 PI minor resistance mutations （41.67%） from 12 PR genes results. [Conclusion] HIV-1 genotyping is an effective tool for the surveillance of HIV-1 drug resistant strains and the testing results confirm that there are HIV-1 drug resistant strains among HIV-1 infected persons without antiretroviral therapy in Shenzhen.