重组人脑利钠肽对大鼠心肌梗死后心功能的影响

EFFECTS OF RHBNP ON CARDIAC FUNCTION AFTER ACUTE MYOCARDIAL INFARCTION

[目的]探讨重组人脑利钠肽(rhBNP)连续给药4周对心肌梗死后大鼠心功能的影响。[方法]制急性心肌梗死(AMI)模型雄性SD大鼠45只,随机分AMI对照组、小剂量rhBNP治疗组、大剂量rhBNP治疗组,各设15只。另设15只作假手术组。rhBNP治疗组经颈静脉输液管输注rhBNP,剂量为15μg/kg,每日1次,持续4周,假手术组及AMI对照组仅以等体积的生理盐水输注。4周后检测血流动力学参数。[结果]与假手术组比较,AMI对照组收缩压(SBP)、舒张压(DBP)、左室收缩压(LVSP)及左室内压最大上升和下降速率(±dp/dt)均明显降低(P均﹤0.01);左室舒张末压(LVEDP)明显升高(P﹤0.01)。与AMI对照组比较,rhBNP治疗组SBP、DBP、LVSP及±dp/dt均明显升高(P均﹤0.01);LVEDP均明显降低(P﹤0.01)。[结论]外源性的重组人脑利钠肽连续应用能改善AMI大鼠血流动血参数,保护心功能。

[Objective] To assess the effects of long-term infusion of recombinant human brain natriuretie peptide （rhBNP） on cardiac function after acute myocardial infarction （AMI） in ratS. [ Methods] Forty-five male Sprague Dawley rats surviving AMI, which was achieved by ligating coronary artery, were randomized to AMI control group （15） or rhBNP treatment group （ 15 ）. Fifteen sham-operated Sprague Dawley rats were selected to serve as non-infarction controls. Two days after surgery, intravenous infusion of rhBNP Or saline （for AMI control and sham-operated groups） was started through jugular vein. After 4 weeks treatment, hemodynamic measurement was performed. [ Results] Comparing with sham-operated rats, SBP, DBP, the left ventricular systolic pressure （LVSP） and the maximum rising and dropping rates of left ventrieular pressure （± dp/ dt） in AMI control group were significandy reduced （all P 〈 0.01） , while the left ventricular end diastolic pressure （LVEDP）, in AMI control group were significantly increased （all P 〈 0.01 ）. In contract, comparing with AMI control group, SBP, DBP, LVSP and ±dp/dt in rhBNP treatment group was significandy increased （all P 〈 0,01）, while LVEDP in rhBNP treatment group was significantly reduced （P 〈 0.01 ）. [Conclusion] RhBNP is effective in improving cardiac function after AMI in rats.