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抗激活素受体相互作用蛋白1抗体的制备及应用 被引量：3

Preparation and application of polyclonal antibody against ARIP1
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摘要 目的:制备抗激活素受体相互作用蛋白1(ARIP1)抗体并探讨其应用。方法:在大肠杆菌BL21中表达GST-ARIP1融合蛋白,以该蛋白作为抗原免疫新西兰家兔,制备抗ARIP1的多克隆抗体。应用该抗体进行免疫组织化学染色,分析ARIP1在小鼠脑组织的分布。结果:制备的兔抗ARIP1多克隆抗体可以特异识别ARIP1,而与ARIP2无交叉反应。初步应用制备的抗体进行免疫组织化学染色,ARIP1在小鼠大脑组织主要表达在下丘脑及海马。结论:成功地制备了抗ARIP1多克隆抗体,该抗体可用于ARIP1成熟蛋白表达的免疫组织化学染色分析。 Objective To prepare anti-activin receptor-interacting protein 1 （ARIP1） polyclonal antibody. Methods The GST-ARIP1 fusion protein was expressed in E. coli BL21. A polyclonal antibody against ARIP1 was obtained by immunizing a rabbit with the purified GST-ARIP1 and the localization of mature ARIP1 protein in mouse brain tissues was detected by immunohistochemistry using the prepared anti-ARIP1 antibody. Results Anti-ARIP1 polyclonal antibody could bind specifically with recombinant ARIP1, but not recombinant ARIP2. Immunohistochemical staining showed that ARIP1 mainly expressed in hypothalamus and hippocampus using the prepared anti-ARIP1 antibody. Conclusion The polyclonal antibody against ARIP1 has been successfully prepared and can be used to do immunohistochemical analysis for ARIP1 protein expression.
作者 方琳 刘海岩 崔雪玲 葛敬岩 柳忠辉
机构地区 吉林大学基础医学院免疫学教研室
出处 《吉林大学学报（医学版）》 CAS CSCD 北大核心 2008年第1期11-13,F0002,共4页 Journal of Jilin University：Medicine Edition
基金 国家自然科学基金资助课题(30170298 30440044)
关键词 抗激活素受体相互作用蛋白1 融合蛋白 抗体 activin receptor-interacting protein 1 fusion protein antibody
分类号 R392 [医药卫生—免疫学]
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共引文献4

同被引文献70

  • 1张红军,台桂香,杨清,柳忠辉.激活素在肝纤维化形成中的作用[J].临床肝胆病杂志,2007,23(3):232-234. 被引量：8
  • 2郑莉琴,戴银清,张彦定.牙齿发育与再生[J].组织工程与重建外科杂志,2007,3(1):1-6. 被引量：8
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吉林大学学报（医学版）
2008年 第1期

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