人参皂苷单体Rh_2对小鼠前胃癌MFC细胞的增殖抑制作用

Anti-proliferation effect of Ginseng Rh_2 on MFC cells

目的:探讨Ginseng Rh2(G-Rh2)对小鼠前胃癌MFC细胞的增殖抑制作用及其机制。方法:MFC细胞(密度为1.0×109·L-1)分为空白组、G-Rh2组(3、10、30mg·L-1)组,MTT法检测MFC细胞活性;流式细胞仪检测细胞周期;流式细胞仪和免疫细胞化学法检测细胞周期蛋白cyclinD1、细胞周期依赖激酶(CDK)抑制蛋白p27kip1在MFC细胞中的定性和定量表达。结果:与相应空白组比较,G-Rh2(3、10、30mg·L-1)组在1、2和4h细胞增殖活性随着剂量和时间增加而逐渐下降(P<0.05或P<0.01);与空白组比较,各药物处理组G0/G1期细胞比率随着剂量增加而上升(P<0.05或P<0.01),同时细胞周期蛋白cyclinD1含量逐渐降低(P<0.05或P<0.01),细胞周期抑制蛋白p27kip1含量逐渐升高(P<0.05或P<0.01)。结论:人参皂苷单体G-Rh2可抑制MFC细胞增殖,可使MFC细胞周期阻滞在G0/G1期而抑制细胞活性,这一过程可能与p27kip1升高、细胞周期蛋白cyclinD1含量降低有关。

Objective To investigate the anti-proliferation effect of Ginseng Rh2 （G-Rh2） on mouse MFC gastric cells and its mechanism, Methods MFC cells （1.0×10^9 · L^-1） were divided into four gourps： control group and G-Rh2 （3, 10, 30 mg· L^-1） groups. The cell viability was determined by MTT; the cell cycle was detected by flow cytometry; the protein expression of cyclin D2 and p27kip1 were observed respectively by qualitative and quantitative analysis. Results Compared with control group, the cell viabilities decreased gradually with the increasing of dose and time in G-Rh2 （3, 10, 30 mg· L^-1） groups at different time （1, 2, 4 h） （P〈0.05 or P〈0.01）, the ratio of VCM Go/G1 cells increased in a dose-dependent manner （P〈0.05 or P〈0.01）, at the same time, the expression level of cell cycle protein cyclin D1 decreased （P〈0.05 or P〈0.01）, and the expression level of p27kip1 increased （P〈0. 05 or P〈0.01）. Conclusion G-Rh2 can decease cell viability by inducing apoptosis and arresting cell cycle, and the process may associate with p27kip1 increase and eyclin D1 decrease.