毛冬青甲素对兔颈总动脉球囊损伤后炎性因子IL-6和M-CSF的影响

Effects of ilexonin A on IL-6 and M-CSF following ballon angioplasty in rabbit common carotid artery

目的:观察毛冬青甲素(Ilexonin,IA)对兔颈总动脉球囊损伤后炎性因子IL-6、M-CSF的影响,为冠脉内介入治疗提供实验依据。方法:日本大耳白兔30只,高胆固醇饲料喂养4周后随机分为3组(每组10只):对照组,右颈外动脉切开后直接缝合切口;球囊扩张组,右颈外动脉切开并内膜球囊损伤后缝合动脉与伤口;IA治疗组,操作同球囊扩张组,缝合切口后静脉给予IA。三组实验兔继续高胆固醇饮食4周。术前1d,术后1d、1周、2周、4周采集血清,放免法测定白细胞介素-6(IL-6)、巨噬细胞集落刺激因子(M-CSF)。取损伤血管段,病理观察动脉病理改变。结果:①球囊扩张组术后血清IL-6水平明显高于IA治疗组(P<0.05),且较IA治疗组恢复延迟;②IA治疗组术后血清M-CSF水平虽增高,但低于球囊扩张组(P<0.05),且恢复术前血清水平时间也较球囊扩张组明显提前(P<0.05);③血管内皮病理变化:颈动脉损伤后对照组内膜光滑,未见泡沫细胞;球囊扩张组内膜增厚,血管平滑肌细胞增生,可见较多泡沫细胞,管腔狭窄;IA治疗组较球囊扩张组泡沫细胞减少,管腔狭窄程度明显减轻。结论:IA能降低动脉内膜球囊损伤后炎性因子IL-6、血清M-CSF水平,并抑制被损伤内膜血管平滑肌细胞增生。

Objective To observe the effects of ilexonin A （IA） on IL-6 and M-CSF following ballon angioplasty in rabbit common carotide artery to provide experimental basis for percutaneous coronary interventions. Methods 30 Japanese rabbits were fed with high cholesterol food for 4 weeks. Then they were divided into three groups randomly. Each group had ten rabbits. ①Control group： the incision was sew directly after right carotide artery of the rabbit was seeked. ②Balloon dilation group： the proximal of the carotide artery was cured, the ballon was delivered and distended, after it was drawn repeatly, the incision was closed. ③IA therapy group： operation was the same to the balloon dilation group, then IA was administered in vein. All of them were fed with high cholesterol diet for 4 weeks and the blood samples were collected 1 d before the operation and 1 d, 1, 2, 4 weeks after the operation. The serum IL-6 and M-CSF levels were determined with radioimmunoassay. The pathological changes of injuried artery were observed. Results ①The IL-6 level in balloon dilation group was higher than that in IA therapy group after the operation （P〈0. 05）, and came back later to preoperation level. ②Though the M-CSF level in IA therapy group was increased, but it was lower than that in dilation group （P〈0.05） and the time of returning to preoperation level was obviously earlier than that in balloon dilation group （P 〈 0.05）. ③ The pathological results demonstrated that the artery endothelial in control group was smooth and no foam cell in sight.In bolloon dilation group, the endangium was thickened, many foam cells and the lumen was constrictive. In IA constrictive degree of lumen was alleviated. Conclusion the vascular smooth muscle cells proliferated, there were therapy group, the number of foam cells reduced, the IA can redece the levels of serum IL-6 and M-CSF and inhibit the proliferation of vascular smooth muscle cells following ballon angioplasty .