摘要
米糠蛋白酶解液分别用截留分子量为10ku和3ku的超滤膜进行超滤,得到分子量10ku以上、3ku～10ku和3ku以下3个级分的ACEI活性肽,经测定其IC50分别为2.95、1.73和0.83g/L,即分子量小于3ku的ACEI活性肽具有最好的ACE抑制效果。研究了分子量小于3ku的ACEI活性肽的稳定性,发现该分子量段的ACEI活性肽有良好的耐热和耐酸碱性,对胃蛋白酶没有耐受性,对胰蛋白酶有良好的耐受性。
The hydrolysate of rice bran protein prepared with proteinase D, was filtrated by ultra filtration membranes （MW 10 ku and 3 ku）.Peptides of MW〉10 ku, MW3 ku -10 ku and MW〈3 ku were obtained and IC50 were 2.95 g/L, 1.73 g/L and 0.83g/L, respectively. It is showed that the peptide below 3 ku has the best ACE inhibitory activity. The stability of ACEI peptide, lower than 3 ku ,was investigated and the results suggested that this kind of peptide could remain activity under acid, alkaline conditions and under hot environment and could have the ability to resist trypsinl enzymes proteolysis. However, the activity decreased when treated by pepsin.
出处
《食品研究与开发》
CAS
北大核心
2008年第9期48-51,共4页
Food Research and Development
基金
江苏省农业攻关项目(BE2005326)
