精氨酸修饰的磁性纳米基因载体的研究

Study on arginine modifying magnetic nanoparticles as gene vector

采用多层修饰方法制备出精氨酸修饰的磁性纳米基因载体，对样品进行了粒度分析、Zeta电位分析、耐酸性能、生物相容性、体外细胞转染性能研究。结果显示，精氨酸修饰的磁性纳米颗粒均匀，分散性好，大多呈圆球型，平均粒径为15nm，粒径范围10～20nm，其Zeta电位为＋29．3mV；多层修饰的纳米磁粒在pH2．0的酸中具有很好的耐酸性能，而未处理的纳米粒子在酸溶液中发生了溶解。MTT实验结果显示，纳米颗粒与细胞有良好的生物相容性；体外细胞转染的结果表明，精氨酸修饰的纳米磁粒能介导pEGFP-N1质粒转染HepC2细胞，并在细胞中表达绿色荧光蛋白。因此，精氨酸修饰的磁性纳米颗粒作为一种新型非病毒基因载体具有介导核酸类生物大分子的应用价值。

An arginine modifying magnetic nanoparticles （AMMN） as gene vector was synthesized by multilay-modifying technique. The AMMN was investigated using granularity analysis, Zeta-potential measurement, acid resistance, biocompatibility and in-vitro transfection. The results showed the morphology of AMMN was mostly spherical and well-distributed,the mean diameter of the nanoparticles was 15 nm and the diameter ranged from 10 ～ 20 nm and its Zeta potential was about ＋ 29.3 mV. These nanoparticles had tremendous durability in acid solution at pH 2.0 while the untreated particles are corroded in acid solution. MTT assay showed the nanoparticles had excellent biocompatibility with cells. The in vitro transfection of AMMN was efficient in HepG2 cells and the expression of green fluorescent proteins was observed under fluorescent microscope. The AMMN may be served as a new-type and effective non-viral carrier for delivery of nueleotides into cells.