用RP-HPLC法测定环合酯中的1-环丙基-6,7-二氟-1,4-二氢-8-甲氧基-4-氧代-3-喹啉羧酸乙酯的含量

RP-HPLC determination of 1-cyclopropyl-6,7-difluoro-8-methyl-1,4-dihydro-4-oxoquinoline-3-ethyl carboxylate in its preparations

建立了以甲醇-水为流动相，使用C18柱进行分离的反向液相色谱法测定环合酯中的1-环丙基-6，7-二氟-1，4-二氢-8-甲氧基4-氧代-3-喹啉羧酸乙酯含量的分析方法，检测波长为254nm，甲醇与水的比例为80：20，柱温为40℃。方法的RSD=0．85％，加标回收率为98．0％～99．8％，最低检测限为0．06μg／mL。该方法操作简便、快速、准确。

ARP-HPLC method for the determination of 1-cyclopropyl-5,7-difluoro-8-methyl-1,4-dihydro-4-oxoquinoline-3-ethyl carboxylate in its preparations was developed using C18 column. The mobile phase consisted of methanol and water （80 ： 20） and the column temperature was 40℃. The detection limit was 0.06μg/mL. The detection wavelength was 254 nm, the recovery was 98.0% ～ 99.8%, the RSD was 0.85% ,the method is simple,fast and accurate.